Recombinant 2019-nCoV NSP8 Protein (His Tag)

    • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience
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    • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience
    • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience

      Catalog number:PKSR030470

      Synonyms:SARS-CoV 2 nsp8

      Size:
      • 10µg
      • 50µg
      Qty:
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      Price: $220

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      Overview

      Synonyms SARS-CoV 2 nsp8
      Species Virus
      Expression_host E.coli
      Sequence Ala1-Gln198
      Accession YP_009725304.1
      Mol_Mass 25 kDa
      AP_Mol_Mass 25 kDa
      Tag C-6His

      Properties

      Purity > 95 % as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per µg as determined by the LAL method.
      Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
      Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
      Formulation Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.
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