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Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag)

Uniprot : YP_009724397.2
  • Cat.No.:PKSR030512

  • Expression host: Baculovirus-Insect Cells

To Purchase PKSR030512

Size:
  • 20µg
Price: $855
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Description

Synonyms NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Species virus
Expression_host Baculovirus-Insect Cells
Sequence (Met1-Ala419(335Gly/Ala))
Accession YP_009724397.2
Mol_Mass 47.08 kDa
Tag C-His

Properties

Purity > 85 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.
Reconstitution Please refer to the printed manual for detailed information.

Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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