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Recombinant 2019-nCoV Papain-Like Protease Protein (Active)

  • Cat.No.:PKSR030472

  • Expression host: E.coli

To Purchase PKSR030472

Size:
  • 10μg
  • 50μg
Price: $220
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Description

Synonyms Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
Species Virus
Expression_host E.coli
Sequence Glu1564-Lys1878
Accession QHD43415.1
Mol_Mass 35.8 kDa
AP_Mol_Mass 34 kDa
Bio_Activity Measured by its ability to cleave a fluorogenic peptide substrate, (EEDANS)RELNGGAPI(K-DABCYL)S (Synthetic).The specific activity is>2000pmoi/min/mg.

Properties

Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Formulation Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.
Reconstitution Please refer to the printed manual for detailed information.

Background

Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.

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