Recombinant AK2/Adenylate kinase 2 Monoclonal Antibody (AN300118P)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela, CHO-K1, C6, mouse Brain, Ramos, HepG2, Rat kidney, Mouse heart in IHC: Human tonsil in IF: HeLa |
| Dilution | WB: 1:1000-1:5000；IHC-P: 1:100-1:200；ICC/IF: 1:200-1:500；IP: 1:10-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF, IP |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human AK2 / Adenylate kinase 2 Protein |
| Abbre | AK2 |
| Synonyms | ADK, KAD, Adenylate kinase isoenzyme, ATP-AMP transphosphorylase, AK2, ADK2, Adenylate kinase 2, Adenylate kinase isoenzyme 2, AK 2, ATP AMP transphosphorylase, ATP-AMP transphosphorylase 2, EC 2.7.4.3, KAD2, mitochondrial, mitochondrial |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
27 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Metabolism, Cancer |
| Clone | A1232 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010] |
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