Recombinant ARL2BP/BART1 Monoclonal Antibody (AN300450P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 in IF: HepG2 |
| Dilution | WB 1:1000-1:5000, IHC-P 1:50, ICC/IF 1:100, FC 1:100-1:200, IP 1:50 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF, FC, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ARL2BP/BART1 protein |
| Abbre | ARL2BP |
| Synonyms | ARL2BP, BART, BART1, RP66, ADP ribosylation factor like 2 binding protein, ADP-ribosylation factor-like protein 2-binding protein, AR2BP, Arf like 2 binding protein BART1, ARF-like 2-binding protein, ARL2 binding protein, ARL2BP protein, Binder of ARF2 protein 1, Binder of Arl Two, Binder of Arl2, Retinitis pigmentosa 66 (autosomal recessive) |
| Swissprot | |
| Calculated MW | 19 kDa |
| Observed MW |
19 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed in retina pigment epithelial cells (at protein level). Widely expressed. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Affinity Purification |
| Research Areas | Signal Transduction |
| Clone | B376 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity. [provided by RefSeq, Jul 2008] |
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