Recombinant CD103 Monoclonal Antibody (AN301460L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse spleen, Mouse lymph node, Rat lymph node |
| Dilution | WB 1:1000, IHC-P 1:500-1:5000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant protein of human CD103 |
| Abbre | CD103 |
| Synonyms | ITGAE, CD103, HUMINAE, HML-1, &, alpha-E integrin-n, aIEL, A530055J10, alpha E, alpha E (antigen CD103, alpha polypeptide), alpha subunit, alpha-E1, alpha-M290, aM290, Antigen CD103, CD 103, CD103 antigen, epithelial-associated, HML 1 antigen, HML1 antigen, HML-1 antigen, Human mucosal lymphocyte antigen 1, Integrin, Integrin alpha E, Integrin alpha E heavy chain, Integrin alpha E light chain, Integrin alpha E1, Integrin alpha IEL, Integrin alpha M290, Integrin alpha-E heavy chain, Integrin alpha-IEL, ITAE, MGC141996, MGC183601, Mucosal lymphocyte 1 antigen, Mucosal lymphocyte antigen 1 |
| Swissprot | |
| Calculated MW | 130 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Immunology, Stem Cells |
| Clone | C121083 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. |
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Videos
