Recombinant CD171/N-CAML1/L1CAM Monoclonal Antibody (AN300178P)
For research use only.
| Verified Samples |
Verified Samples in WB: A375 in IHC: Human kidney |
| Dilution | IHC 1:200-1:1000, WB 1:500-1:2000, IF 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human CD171 / N-CAML1 / L1CAM protein |
| Abbre | L1CAM |
| Synonyms | MIC, SPG, CAML, N-CAML, NCAM-L, N-CAM-L, L1CAM, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1, N-CAM L1, L1 antigen, CAML1, CD171, HSAS, HSAS1, L1cam, MASA, MIC5, S10, SPG1, Antigen identified by monoclonal antibody R1, Antigen identified by monoclonal R1, CD171 antigen, Hyd, L1 cell adhesion molecule, L1-NCAM, N CAML1, NCAML1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, OTTHUMP00000025992 |
| Swissprot | |
| Calculated MW | 140kD |
| Observed MW |
250kD
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Cell projection, Growth cone, Axon, Dendrite. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Neuroscience |
| Clone | A1292 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013] |
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