Recombinant CD86/B7-2 Monoclonal Antibody (AN300007P)

Name: Recombinant CD86/B7-2 Monoclonal Antibody
Brand: Elabscience
SKU: AN300007P
Price: 150.0 USD
Availability: InStock

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Recombinant CD86/B7-2 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	20μL	$ 150.00	- +

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Verified Samples	Verified Samples in WB:Raji
Dilution	WB: 1:1000；ICC/IF: 1:100；FC: 1:200-1:500；IP: 1:20-1:50
Isotype	IgG
Host	Rabbit
Reactivity	Human
Applications	WB, ICC/IF, FC, IP
Clonality	Recombinant;Monoclonal
Immunogen	Recombinant Human CD86 / B7-2 Protein
Abbre	CD86
Synonyms	LAB, ETC, FUN, CTLA-4 Counter-Receptor B, Early T-cell costimulatory molecule, CD28LG, B7-2, B7.2, B70, CD28LG2, LAB72, CD86, Activation B7-2 antigen, BU63, CTLA-4 Counter-Receptor B7.2, Early T-cell costimulatory molecule 1, ETC-1, FUN-1, T-lymphocyte activation antigen CD86, B7-2, Activation B7-2 antigen 3, B7 2, B72 antigen, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD86 antigen, CD86 molecule, CLS1, CTLA-4 counter-receptor B7.2 2, FUN 1, Ly58, Ly-58, MB7, MB7-2, Membrane glycoprotein, MGC34413, TS/A-2
Swissprot	P42081
Calculated MW	38 kDa
Observed MW	60-85 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cell membrane, Secreted.
Concentration	1 mg/mL
Buffer	0.2 μm filtered solution in PBS
Purification Method	Protein A
Research Areas	Immunology, Microbiology, Stem Cells, Kits, Lysates, Other
Clone	A1117
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]