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Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)

Uniprot : P05804

Cat.No.:PKSQ050059
Expression host: E.coli

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Description

Synonyms	Beta-glucuronidase;uidA;GUS
Species	E.coli
Expression_host	E.coli
Sequence	Met1-Gln603
Accession	P05804
Mol_Mass	69.9 kDa
AP_Mol_Mass	72 kDa
Tag	N-His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation	Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Reconstitution	Not Applicable

Background

Beta-D-glucuronidase from E.coli is a ?highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.

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