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Recombinant Human ACE2 Protein (Fc Tag)(Active)

Uniprot : NP_068576.1
  • Cat.No.:PKSH030457

  • Expression host: HEK293 Cells

To Purchase PKSH030457

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  • 20µg
  • 100µg
Price: $695
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Description

Synonyms Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2
Species Human
Expression_host HEK293 Cells
Sequence Met 1-Ser 740
Accession NP_068576.1
Mol_Mass 110.3 kDa
AP_Mol_Mass 145-150 kDa
Tag C-Fc
Bio_Activity 1.Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 6 nM.2. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) was 30 nM.3. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 30 nM.4. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc bound to Spike-His (aa 1-760) was 10 nM.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Reconstitution Please refer to the printed manual for detailed information.

Background

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.

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