Recombinant Human ATG3 Protein (PKSH032098)
For research use only.
| Synonyms | APG3, APG3-like, APG3L, ATG3, Autophagy-related protein 3, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3, hApg3 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Met314 |
| Accession | Q9NT62 |
| Calculated Molecular Weight | 36.0 kDa |
| Observed Molecular Weight | 38 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
| Background | Ubiquitin-like-conjugating enzyme ATG3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. As an E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. ATG3 also can catalyze the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy. |
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