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Recombinant Human β-NGF/NGFB Protein

Cat:PKSH033269
Manual MSDS

Price: $ 249

Price: $ 98

Size:
100μg 20μg
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  • Expression Host: E.coli
  • Accession: P01138
  • Applications: Cell culture
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Product Details
Synonyms Beta-Nerve Growth Factor;Beta-NGF;NGF;NGFB
Species Human
Expression Host E.coli
Sequence Ser122-Ala241
Accession P01138
Calculated Molecular Weight 14.4 kDa
Observed Molecular Weight 11 kDa
Tag C-His
Bio-activity Measure by its ability to induce TF-1 cells proliferation. The ED<sub>50</sub> for this effect is <0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 x 10<sup>6</sup> IU/mg.
Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.