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Recombinant Human β-NGF/NGFB Protein (with pro peptide)

Uniprot : P01138

Cat.No.:PKSH033552
Expression host: E.coli

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Description

Synonyms	Beta-Nerve Growth Factor;Beta-NGF;NGF;NGFB
Species	Human
Expression_host	E.coli
Sequence	Glu19-Ala241
Accession	P01138
Application	Cell Culture
Mol_Mass	25 kDa
AP_Mol_Mass	30 kDa
Tag	None

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,500mM NaCl,5%Trehalose,5%Mannitol,0.01%tween80,1mM EDTA,pH8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to th
Reconstitution	Please refer to the printed manual for detailed information.

Background

The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges; whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.

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