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Recombinant Human Calmegin/CLGN Protein (His Tag)

Uniprot : O14967
  • Cat.No.:PKSH032147

  • Expression host: HEK293 Cells

To Purchase PKSH032147

Size:
  • 10μg
  • 50μg
Price: $178
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Description

Synonyms Calmegin;CLGN
Species Human
Expression_host HEK293 Cells
Sequence Glu20-Trp471
Accession O14967
Mol_Mass 52.9 kDa
AP_Mol_Mass 60 kDa
Tag C-His
Bio_Activity Not validated for activity

Properties

Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Calmegin (CLGN) is a member of the calreticulin family. Calmegin is a testis-specific endoplasmic reticulum chaperone protein. The functions during spermatogenesis as a chaperone for a range of client proteins that is important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. It is required for normal male fertility, normal sperm migration from the uterus into the oviduct. Calmegin binds calcium ions and interacts with PDILT. Calmegin may play a role in spermatogeneisis and infertility.

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