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Recombinant Human Carbonic Anhydrase 10/CA10 Protein

Uniprot : Q9NS85

Cat.No.:PKSH031484
Expression host: HEK293 Cells

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20μg

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Description

Synonyms	Carbonic Anhydrase-Related Protein 10;Carbonic Anhydrase-Related Protein X;CA-RP X;CARP X;Cerebral Protein 15;CA10;CA-RPX;CARPX;HUCEP-15
Species	Human
Expression_host	HEK293 Cells
Sequence	Met 1-Asn 300
Accession	NP_001076002.1
Mol_Mass	27.2 kDa
AP_Mol_Mass	38 kDa
Tag	None
Bio_Activity	Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

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