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Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)

Uniprot : Q13231
  • Cat.No.:PKSH032245

  • Expression host: HEK293 Cells

To Purchase PKSH032245

Size:
  • 10μg
  • 50μg
Price: $223
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Description

Synonyms Chitotriosidase-1;Chitinase-1;CHIT1;CHI3;CHIT;CHITD
Species Human
Expression_host HEK293 Cells
Sequence Ala22-Asn466
Accession AAI03696.1
Mol_Mass 50.4 kDa
AP_Mol_Mass 54 kDa
Tag C-His

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
Reconstitution Not Applicable

Background

Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly, respectively.

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