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Recombinant Human CLIC1 Protein (His Tag)

Uniprot : O00299
  • Cat.No.:PKSH032246

  • Expression host: E.coli

To Purchase PKSH032246

Size:
  • 10μg
  • 50μg
Price: $117
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Description

Synonyms Chloride Intracellular Channel Protein 1;Chloride Channel ABP;Nuclear Chloride Ion Channel 27;NCC27;Regulatory Nuclear Chloride Ion Channel Protein;hRNCC;CLIC1;G6;NCC27
Species Human
Expression_host E.coli
Sequence Met 1-Lys241
Accession O00299
Mol_Mass 29.0 kDa
AP_Mol_Mass 30-35 kDa
Tag N-His
Bio_Activity Not validated for activity

Properties

Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Chloride Intracellular Channel Protein 1 (CLIC1) belongs to the Chloride Channel CLIC family and contains one GST C-terminal domain. CLIC1 can be expressed in various cell types, but it is especially prominent in the heart, placenta, liver, kidney, and pancreas. CLIC1 can insert into membranes and form chloride ion channels. The channel activity depends on the pH. CLIC1 membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. CLIC1 is also involved in the regulation of the cell cycle.

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