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Recombinant Human IGF-1/IGF1 Protein (aa 52-118)

Cat:PKSH032595
Manual MSDS

Price: $ 55

Price: $ 31

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50μg 10μg
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  • Expression Host: E.coli
  • Accession: P05019
  • Applications: Cell culture
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Product Details
Synonyms Insulin-Like Growth Factor I;IGF-I;Mechano Growth Factor;MGF;Somatomedin-C;IGF1;IBP1
Species Human
Expression Host E.coli
Sequence Thr52-Ala118
Accession P05019
Calculated Molecular Weight 7.3 kDa
Observed Molecular Weight 9 kDa
Tag None
Bio-activity Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED<sub>50</sub> for this effect is 20-100 ng/ml.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 0.5 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.