Recombinant Human IL1F5/IL36RN Protein
Price: $ 739
Price: $ 249
Size:
100μg
20μg
Quantity:
- Expression Host: E.coli
- Accession: Q9UBH0
- Applications: Cell culture
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Product Details
Synonyms | Interleukin-36 Receptor Antagonist Protein;FIL1 Delta;IL-1-Related Protein 3;IL-1RP3;Interleukin-1 HY1;IL-1HY1;Interleukin-1 Delta;IL-1 Delta;Interleukin-1 Family Member 5;IL-1F5;Interleukin-1 Receptor Antagonist Homolog 1;IL-1ra Homolog 1;Interleukin-1-Like Protein 1;IL-1L1;IL36RN;FIL1D;IL1F5;IL1HY1;IL1L1;IL1RP3 |
Species | Human |
Expression Host | E.coli |
Sequence | Met 1-Asp155 |
Accession | Q9UBH0 |
Calculated Molecular Weight | 17.8 kDa |
Observed Molecular Weight | 17 kDa |
Tag | C-His |
Bio-activity | Measure by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells.The ED<sub>50</sub> for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Interleukin-1 family member 5 (IL-1F5); also known as interleukin 36 receptor antagonist (IL36RA); is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family; member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A); beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1); which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor; single Ig IL-1R-related molecule (SIGIRR)/TIR8; as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue; IL-1F5 did not attenuate LPS-induced changes; or up-regulated IL-4 in macrophages or dendritic cells; suggesting that the effect is confined to the brain. |