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Cat.No.:PKSH031854
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Synonyms | Interleukin-1 Receptor Antagonist Protein;IL-1RN;IL-1ra;IRAP;ICIL-1RA;IL1 Inhibitor;Anakinra;IL1RN;IL1F3;IL1RA |
Species | Human |
Expression_host | E.coli |
Sequence | Arg 26-Glu 177 |
Accession | NP_776214.1 |
Application | Functional ELISA |
Mol_Mass | 17.3 kDa |
AP_Mol_Mass | 17.3 kDa |
Tag | None |
Bio_Activity | 1. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R2-Fc, The EC50 of human IL1R2-Fc is 0.04-0.1 μg/mL. 2. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R1-Fc, The EC50 of human IL1R1-Fc is 0.08-0.2 μg/mL. 3. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 250pg/mL IL1a. The EC50 for this effect is typically 3-12 ng/mL. |
Purity | > 97 % as determined by reducing SDS-PAGE. |
Endotoxin level | Please contact us for more information. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |