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Recombinant Human Interleukin-7/IL-7 Protein

Cat:PKSH033610
Manual MSDS

Price: $ 739

Price: $ 249

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100μg 20μg
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  • Expression Host: E.coli
  • Accession: P13232
  • Applications: Cell culture
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Product Details
Synonyms Interleukin-7;IL-7;IL7
Species Human
Expression Host E.coli
Sequence Asp26-His177
Accession P13232
Calculated Molecular Weight 18.3 kDa
Observed Molecular Weight 19 kDa
Tag C-His
Bio-activity Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED<sub>50</sub> for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10<sup>8</sup> IU/mg.
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.