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Cat.No.:PKSH031969
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Synonyms | Macrophage Colony-Stimulating Factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1, SCF-1 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Asn190 |
Accession | NP_757349.1 |
Application | Cell Culture |
Mol_Mass | 18.4 kDa |
Tag | None |
Bio_Activity | Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1. 6-6. 3 ng/mL. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4. 5% Trehalose, 5% Mannitol, 0.01% Tween-80 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed |
Reconstitution | Please refer to the printed manual for detailed information. |
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y**aSubmitted [ Aug 04 2023 ]
Asked: Hi, in the reconstruction procedure in the printed manual instructed to prepare a stock solution of 0.25 mg/ml from 20 μg vial
Reply
adminSubmitted [ Aug 04 2023 ]
Answered:
adminSubmitted [ Aug 04 2023 ]
Answered: It is recommended that sterile water (80ul) be added to the vial (20ug) to prepare a stock solution of 0.25mg/mL.