Recombinant Human MAPKAPK5 Protein (His & GST Tag)
Price: $ 717
Size:
50μg
Quantity:
- Expression Host: Baculovirus-Insect Cells
- Accession: NP_003659.2
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For research use only. Order now, ship in 3 days
Product Details
Synonyms | MAPKAP-K5;MK-5;MK5;PRAK |
Species | Human |
Expression Host | Baculovirus-Insect Cells |
Sequence | Met 1-Gln 471 |
Accession | NP_003659.2 |
Calculated Molecular Weight | 82.0 kDa |
Observed Molecular Weight | 75 kDa |
Tag | N-His-GST |
Purity | > 76 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol |
Reconstitution | Not Applicable |
Background | MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation. |