Recombinant Human MAPKAPK5 Protein (His & GST tag)

    • Recombinant Human MAPKAPK5 Protein (His & GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Human MAPKAPK5 Protein (His & GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Human MAPKAPK5 Protein (His & GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSH030328

      Synonyms:MAPKAP-K5;MK-5;MK5;PRAK

      Size:
      • 20µg
      • 50µg
      Qty:
      - +
      Price: $486

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms MAPKAP-K5;MK-5;MK5;PRAK
      Species Human
      Expression_host Baculovirus-Insect Cells
      Sequence Met 1-Gln 471
      Accession NP_003659.2
      Mol_Mass 82 kDa
      AP_Mol_Mass 75 kDa
      Tag N-His & GST

      Properties

      Purity > 76 % as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per µg as determined by the LAL method.
      Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
      Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
      Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.

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