Synonyms	Methylated-DNA--protein-cysteine methyltransferase;6-O-methylguanine-DNAmethyltransferase;O-6-methylguanine-DNA-alkyltransferase;MGMT
Species	Human
Expression_host	E.coli
Sequence	Met 1-Asn207
Accession	P16455
Mol_Mass	23.8 kDa
AP_Mol_Mass	25 kDa
Tag	N-His

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100,pH 8.0.
Reconstitution	Not Applicable

Background

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Citations

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