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Recombinant Human Noggin/NOG Protein (aa 28-232, Fc Tag)(Active)

  • Cat.No.:PKSH033577

  • Expression host: Human Cells

To Purchase PKSH033577

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  • 10μg
  • 50μg
Price: $90
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Description

Synonyms Noggin;SYM1;SYNS1
Species Human
Expression_host Human Cells
Sequence Gln28-Cys232
Accession Q13253
Application Functional ELISA
Mol_Mass 50.2 kDa
AP_Mol_Mass 60 kDa
Tag C-Fc
Bio_Activity Immobilized Human BMP-2(Cat: PKSR030454) at 2μg/ml(100 μl/well) can bind Human Noggin-Fc. The ED50 of Human Noggin-Fc is 0.045μg/mL.

Properties

Purity > 95% as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg as determined by the LAL method.
Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

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