Synonyms	Nucleolar Protein 3;Apoptosis Repressor With CARD;Muscle-Enriched Cytoplasmic Protein;Myp;Nucleolar Protein of 30 kDa;Nop30;NOL3;ARC;NOP
Species	Human
Expression_host	E.coli
Sequence	Met 1-Ser208
Accession	O60936
Mol_Mass	48.9 kDa
AP_Mol_Mass	30-59 kDa
Tag	N-GST
Bio_Activity	Not validated for activity

Properties

Purity	> 85 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 350mM NaCl, 1mM DTT, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Nucleolar Protein 3 is encoded by NOL3 gene; multiple transcript variants encoding different isoforms have been found for this gene. So far; Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing.Isoform 2 may inhibit apoptosis.It has been shown to down-regulate the enzyme activities of caspase 2; caspase 8 and tumor protein p53.

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