English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Recombinant Human PFK2/PFKFB3 Protein (His & GST Tag)

Cat:PKSH030329
Manual MSDS

Price: $ 805

Size:
50μg
Quantity:
  • Expression Host: Baculovirus-Insect Cells
  • Accession: Q16875-1
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Synonyms IPFK2;PFK2
Species Human
Expression Host Baculovirus-Insect Cells
Sequence Met 1-His 520
Accession Q16875-1
Calculated Molecular Weight 87.4 kDa
Observed Molecular Weight 75 kDa
Tag N-His-GST
Purity > 85 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.0, 10% glycerol, 0.3mM DTT
Reconstitution Not Applicable
Background Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFK-2 / FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions.