Recombinant Human PKC nu / PRKD3 Protein (GST tag)

    • Recombinant Human PKC nu / PRKD3 Protein (GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Human PKC nu / PRKD3 Protein (GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Human PKC nu / PRKD3 Protein (GST tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSH030402

      Synonyms:EPK2;nPKC-NU;PKC-NU;PKD3;PRKCN

      Size:
      • 20 µg
      • 50 µg
      Qty:
      - +
      Price: $365

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms EPK2;nPKC-NU;PKC-NU;PKD3;PRKCN
      Species Human
      Expression_host Baculovirus-Insect Cells
      Sequence Met 1-Pro 890
      Accession NP_005804.1
      Mol_Mass 126.7 kDa
      AP_Mol_Mass 126.7 kDa
      Tag N-GST

      Properties

      Purity > 85 % as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per µg as determined by the LAL method.
      Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
      Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice.Upon receipt, store it immediately at<-20°C.
      Formulation Supplied as sterile 20mM Tris, 500mM NaCl, 10mM Reduced Glutathione, pH 7.4
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Serine/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD3 / PRKCN contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 / PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 / PRKCN is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 / PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 / PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.

      SDS-PAGE

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