Recombinant Human SCF/c-Kit Ligand Protein
Price: $ 739
Price: $ 249
Size:
100μg
20μg
Quantity:
- Expression Host: E.coli
- Accession: P21583
- Applications: Cell culture
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Product Details
Synonyms | Kit Ligand;Mast Cell Growth Factor;MGF;Stem Cell Factor;SCF;c-Kit ligand;KITLG;MGF;SCF |
Species | Human |
Expression Host | E.coli |
Sequence | Glu26-Ala189 |
Accession | P21583 |
Calculated Molecular Weight | 19.4 kDa |
Observed Molecular Weight | 21 kDa |
Tag | C-His |
Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED<sub>50</sub> for this effect is <5 ng/mL. The specific activity of recombinant human SCF is > 5 x 10<sup>5</sup> IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. |