Recombinant Human SELENOI/EPT1 Protein (GST Tag) (PKSH032407)
For research use only.
| Synonyms | EPT1, Ethanolaminephosphotransferase 1, KIAA1724, SELI, SelI, Selenoprotein I, hEPT1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Pro50 |
| Accession | Q9C0D9 |
| Calculated Molecular Weight | 32.6 kDa |
| Observed Molecular Weight | 29 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.0. |
| Reconstitution | Not Applicable |
| Background | Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway. |
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