Synonyms	SIGLEC10;MGC126774;PRO940;Siglec10;SLG2;sialic acid-binding Ig-like lectin 10;Siglec-10;siglec-like gene 2;Siglec-like protein 2;SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
Species	Human
Expression_host	HEK293 Cells
Sequence	Met17-Thr546
Accession	Q96LC7
Mol_Mass	85.1 kDa
AP_Mol_Mass	110-120 kDa
Tag	C-Fc
Bio_Activity	Immobilized Anti-Human Siglec10 mAb at 2μg/ml (100 μl/well) can bind Human Siglec-10-Fc. The ED50 of Human Siglec-10-Fc is 9.18 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Sucrose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the
Reconstitution	Please refer to the printed manual for detailed information.

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Citations

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