Recombinant Human Sonic Hedgehog/SHH Protein (C24II)(Active)

    • Recombinant Human Sonic Hedgehog / Shh Protein (C24II)-Elabscience
    • Recombinant Protein-Elabscience
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    • Recombinant Human Sonic Hedgehog / Shh Protein (C24II)-Elabscience
    • Recombinant Protein-Elabscience
    • Recombinant Human Sonic Hedgehog / Shh Protein (C24II)-Elabscience
    • Recombinant Protein-Elabscience

      Catalog number:PKSH033518

      Synonyms:Sonic Hedgehog Protein; SHH; HHG-1; SHH

      Size:
      • 10μg
      • 50μg
      Qty:
      - +
      Price: $144

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Overview

      Synonyms Sonic Hedgehog Protein; SHH; HHG-1; SHH
      Species Human
      Expression_host E.coli
      Sequence Cys24-Gly197(Cys24Ile-Ile)
      Accession Q15465
      Application Functional ELISA
      Mol_Mass 19.8 kDa
      AP_Mol_Mass 20 kDa
      Bio_Activity Immobilized Human SHH at 1μg/ml(100 μl/well) can bind Human BOC-His(Cat: PKSH032123).

      Properties

      Purity > 95% as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per μg as determined by the LAL method.
      Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.

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