Synonyms	Sarcoma growth factor;TGF-type I;ETGF
Species	Human
Expression_host	E.coli
Sequence	Val 40-Ala 89
Accession	P01135
Mol_Mass	6.5 kDa
AP_Mol_Mass	7 kDa
Tag	C-His
Bio_Activity	Measure by its ability to induce 3T3 cells proliferation.The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant human TGF alpha is > 5 x 10⁶ IU/mg.

Properties

Purity	> 98 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.1 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS,pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Citations

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