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Cat.No.:PKSH033166
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Synonyms | Lymphotoxin-Alpha;LT-Alpha;TNF-Beta;Tumor Necrosis Factor Ligand Superfamily Member 1;LTA;TNFB;TNFSF1 |
Species | Human |
Expression_host | E.coli |
Sequence | Leu35-Leu205 |
Accession | P01374 |
Application | Cell Culture |
Mol_Mass | 18.7 kDa |
AP_Mol_Mass | 21 kDa |
Tag | N-His |
Bio_Activity | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 108 IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |