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Recombinant Human TNFSF4/OX40L Protein (His Tag)(Active)

Uniprot : P23510

Cat.No.:PKSH032842
Expression host: HEK293 Cells

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Description

Synonyms	Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, OX40L, TAX transcriptionally-activated glycoprotein 1, TNFSF4, CD252, TXGP1, CD134L, CD252, GP34, OX-40, TXGP1
Species	Human
Expression_host	HEK293 Cells
Sequence	Gln51-Leu183
Accession	P23510
Application	Functional ELISA
Mol_Mass	16.3 kDa
AP_Mol_Mass	25-28 kDa
Tag	N-6His
Bio_Activity	Immobilized Human OX40-Fc(Cat: PKSH032844) at 2μg/ml(100 μl/well) can bind Biotinylated Human OX40L-His. The ED50 of Human OX40L-His is 4. 7 ug/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB;150mM NaCl, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manua
Reconstitution	Please refer to the printed manual for detailed information.

Background

Tumor necrosis factor ligand superfamily member 4(TNFSF4/OX40L) is a single-pass type II membrane protein.OX40L is expressed on the surface of activated B cells; T cells; dendritic cells and endothelial cells. OX40L binds to OX40 (CD134); a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40-OX40L co-stimulates signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. It involved in T-cell proliferation and cytokine production. Additional; it has been found association with systemic lupus erythematosus; no association with occurrence of atherosclerosis.

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