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Cat.No.:PKSH031303
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Synonyms | Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;HEL-S-71p;PRO1557;PRO2086;TFQTL1 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Pro 698 |
Accession | NP_001054.1 |
Application | Cell Culture, Functional ELISA |
Mol_Mass | 76.6 kDa |
AP_Mol_Mass | 74 kDa |
Tag | C-His |
Bio_Activity | 1. Immobilized human CD71 at 10 μg/ml (100 μl/well) can bind human Transferrin. The EC50 of human Transferrin is 5. 6 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K. P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 μg/mL. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |