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Recombinant Human Transforming Growth Factor Beta-1/TGFB1

Uniprot : P01137
  • Cat.No.:PKSH033947

  • Expression host: HEK293 Cells

To Purchase PKSH033947

Size:
  • 10μg
  • 50μg
Price: $155
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Description

Synonyms Transforming Growth Factor Beta-1;TGF-Beta-1;Latency-Associated Peptide;LAP;TGFB1;TGFB
Species Human
Expression_host HEK293 Cells
Sequence Leu30-Arg278(Cys33Ser)
Accession P01137
Mol_Mass 28.5 kDa
AP_Mol_Mass 20-30 kDa
Tag None

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide.Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species. Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1.

Citations

Reviews/Q&A

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  • Reviews
  • Q&A
Q

D*****aSubmitted [ Feb 27 2024 ]

Asked: I\'m planning to purchase TGF-B1, product no. PKSH033947, and I was wondering if this product can be used to induce epithelial to mesenchymal transition in lung adnocarcinoma A459 cells ?

Reply

A

adminSubmitted [ Feb 27 2024 ]

Answered: We have not test the bioactivity of PKSH033947, so we are not sure if it is suitable for your experiment. We have another protein that might fit your requirement, please see the info below: PKSH034147 Recombinant Human TGF beta 1 protein(N-His)(active) Bio-activity: 1. Measured by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately >5 x 10⁷ IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.

Q

H********rSubmitted [ Feb 26 2024 ]

Asked: I was wondering if this product is suitable for cell culture to induce epithelial to mesenchymal transition in lung adenocarcinoma epithelial cells, such as the A549 cell line?

Reply

A

adminSubmitted [ Feb 26 2024 ]

Answered: We have not test the bioactivity of PKSH033947, so we are not sure if it is suitable for your experiment. We have another protein that might fit your requirement, please see the info below: PKSH034147 Recombinant Human TGF beta 1 protein(N-His)(active) Bio-activity: 1. Measured by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately >5 x 10⁷ IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.

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