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Cat.No.:PKSH033947
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Synonyms | Transforming Growth Factor Beta-1;TGF-Beta-1;Latency-Associated Peptide;LAP;TGFB1;TGFB |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Leu30-Arg278(Cys33Ser) |
Accession | P01137 |
Mol_Mass | 28.5 kDa |
AP_Mol_Mass | 20-30 kDa |
Tag | None |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
D*****aSubmitted [ Feb 27 2024 ]
Asked: I\'m planning to purchase TGF-B1, product no. PKSH033947, and I was wondering if this product can be used to induce epithelial to mesenchymal transition in lung adnocarcinoma A459 cells ?
Reply
adminSubmitted [ Feb 27 2024 ]
Answered: We have not test the bioactivity of PKSH033947, so we are not sure if it is suitable for your experiment. We have another protein that might fit your requirement, please see the info below: PKSH034147 Recombinant Human TGF beta 1 protein(N-His)(active) Bio-activity: 1. Measured by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately >5 x 10⁷ IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.
H********rSubmitted [ Feb 26 2024 ]
Asked: I was wondering if this product is suitable for cell culture to induce epithelial to mesenchymal transition in lung adenocarcinoma epithelial cells, such as the A549 cell line?
Reply
adminSubmitted [ Feb 26 2024 ]
Answered: We have not test the bioactivity of PKSH033947, so we are not sure if it is suitable for your experiment. We have another protein that might fit your requirement, please see the info below: PKSH034147 Recombinant Human TGF beta 1 protein(N-His)(active) Bio-activity: 1. Measured by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beta 1 is approximately >5 x 10⁷ IU/mg. 2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.