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Recombinant Human UBE2A Protein (His Tag)

Uniprot : P49459
  • Cat.No.:PKSH030787

  • Expression host: E.coli

To Purchase PKSH030787

Size:
  • 100μg
Price: $670
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Description

Synonyms Ubiquitin-Conjugating Enzyme E2 A;RAD6 Homolog A;HR6A;hHR6A;Ubiquitin Carrier Protein A;Ubiquitin-Protein Ligase A;UBE2A;RAD6A
Species Human
Expression_host E.coli
Sequence Met 1-Cys 152
Accession P49459
Mol_Mass 19.2 kDa
AP_Mol_Mass 18.5 kDa
Tag N-His

Properties

Purity > 80 % as determined by reducing SDS-PAGE.
Endotoxin level Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, 20% glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A); encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.

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