Recombinant Mouse CD73/NT5E Protein (His Tag)(Active)

    • Recombinant Mouse  CD73 Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Mouse  CD73 Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Mouse  CD73 Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSM040791

      Synonyms:5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT;AI447961; eNT; NT; Nt5 Protein

      Size:
      • 10 μg
      • 20 μg
      Qty:
      - +
      Price: $454

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms 5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT;AI447961; eNT; NT; Nt5 Protein
      Species Mouse
      Expression_host HEK293 Cells
      Sequence Met 1-Lys 549
      Accession NP_035981.1
      AP_Mol_Mass 59.4 kDa
      Tag C-His
      Bio_Activity Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).The specific activity is >10,000 pmol/mi

      Properties

      Purity > 95 % as determined by SDS-PAGE
      Endotoxin < 1.0 EU per µg of the protein as determined by the LAL method.
      Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from sterile PBS, pH 7.4
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

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      MSDS for ELISA ISO 9001

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