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Cat.No.:PKSM040791
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Synonyms | 5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT;AI447961;eNT;NT;Nt5 Protein |
Species | Mouse |
Expression_host | HEK293 Cells |
Sequence | Met 1-Lys 549 |
Accession | NP_035981.1 |
Mol_Mass | 59.4 kDa |
AP_Mol_Mass | 59.4 kDa |
Tag | C-His |
Bio_Activity | Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is > 10, 000 pmol/min/µg. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |