Synonyms	Cd152;Ctla-4;Ly-56
Species	Mouse
Expression_host	HEK293 Cells
Sequence	Met 1-Phe 162
Accession	NP_033973.2
Application	Functional ELISA
Mol_Mass	15.3 kDa
AP_Mol_Mass	25-30 kDa
Tag	C-His
Bio_Activity	1. Immobilized recombinant mouse CTLA4-His at 10 μg/ml (100 μl/well) can mouse CD80-Fc with a linear range of 0.78-62. 5 ng/ml. 2. Immobilized recombinant mouse CTLA4-His at 10 μg/ml (100 μl/well) can human B7-1-Fc with a linear range of 0.78-125 ng/ml.

Properties

Purity	> 98 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

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