Recombinant Mouse FAS/TNFRSF6 Protein (His Tag)(Active)

    • Recombinant Mouse  Fas Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
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    • Recombinant Mouse  Fas Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience
    • Recombinant Mouse  Fas Protein (His Tag)-Elabscience
    • Recombinant protein products for various applications-Elabscience

      Catalog number:PKSM040905

      Synonyms:AI196731 Protein, APO1 Protein, APT1 Protein, CD95 Protein, lpr Protein, TNFR6 Protein, Tnfrsf6 Protein

      Size:
      • 50 μg
      • 100 μg
      Qty:
      - +
      Price: $454

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms AI196731 Protein, APO1 Protein, APT1 Protein, CD95 Protein, lpr Protein, TNFR6 Protein, Tnfrsf6 Protein
      Species Mouse
      Expression_host HEK293 Cells
      Sequence Met 1-Arg 169
      Accession NP_032013.2
      Mol_Mass 18 kDa
      Tag C-His
      Bio_Activity Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 1-8 µg/mL in the presence of 20 ng/mL recombinant human Fas ligand.

      Properties

      Purity > 98 % as determined by SDS-PAGE
      Endotoxin < 1.0 EU per µg of the protein as determined by the LAL method.
      Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from sterile PBS, pH 7.4
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

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      MSDS for ELISA ISO 9001

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