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Recombinant Mouse GM-CSF/CSF2 Protein

Cat:PKSM041031
Manual MSDS

Price: $ 739

Price: $ 249

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100μg 20μg
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  • Expression Host: E.coli
  • Accession: P01587
  • Applications: Cell culture
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Product Details
Synonyms Granulocyte-macrophage colony-stimulating factor;Csf2;GM-CSF;Colony-stimulating factor;Csfgm
Species Mouse
Expression Host E.coli
Sequence Ala18-Lys141
Accession P01587
Calculated Molecular Weight 15.1 kDa
Observed Molecular Weight 17 kDa
Tag N-His
Bio-activity Measure by its ability to induce proliferation in FDC-P1 cells. The ED<sub>50</sub> for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 10<sup>7</sup> IU/mg.
Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin < 0.1 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.