Recombinant Mouse GM-CSF/CSF2 Protein
Price: $ 739
Price: $ 249
Size:
100μg
20μg
Quantity:
- Expression Host: E.coli
- Accession: P01587
- Applications: Cell culture
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Product Details
Synonyms | Granulocyte-macrophage colony-stimulating factor;Csf2;GM-CSF;Colony-stimulating factor;Csfgm |
Species | Mouse |
Expression Host | E.coli |
Sequence | Ala18-Lys141 |
Accession | P01587 |
Calculated Molecular Weight | 15.1 kDa |
Observed Molecular Weight | 17 kDa |
Tag | N-His |
Bio-activity | Measure by its ability to induce proliferation in FDC-P1 cells. The ED<sub>50</sub> for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 10<sup>7</sup> IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines. |