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Cat.No.:PKSM041325
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Synonyms | IGF1;IGF-1;insulin-like growth factor 1;Insulin-like growth factor I;Somatomedin C;somatomedin-C |
Species | Mouse |
Expression_host | E.coli |
Sequence | Gly49-Ala118 |
Accession | P05017 |
Application | Cell Culture |
Mol_Mass | 8.6 kDa |
AP_Mol_Mass | 11-17 kDa |
Tag | C-His |
Bio_Activity | Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is <2 ng/mL. The specific activity of recombinant mouse IGF-I is > 5 x 105 IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |