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Recombinant Mouse Interleukin-2/IL-2 Protein (Active)

Uniprot : P04351

Cat.No.:PKSM041320
Expression host: E.coli

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Description

Synonyms	IL2, Aldesleukin, Interleukin 2, Interleukin-2, IL-2, IL2, T-cell growth facter, T cell growth factor, TCGF
Species	Mouse
Expression_host	E.coli
Sequence	Ala21-Gln169
Accession	P04351
Application	Cell Culture
Mol_Mass	17.4 kDa
AP_Mol_Mass	17 kDa
Tag	None
Bio_Activity	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The specific activity of Recombinant Mouse IL-2 is ≥1×107 IU/mg.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.01 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate, 0.2%Tween 80,pH3.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the
Reconstitution	Please refer to the printed manual for detailed information.

Background

Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

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