Recombinant Mouse M-CSF protein(His Tag) (PKSM041498)
For research use only.
| Synonyms | Ath, Ath-, Ath-1, Ath1, CD134, CD134L, OX-40L, OX4, TNFSF4, TXGP1, Tnlg2b, Txgp, Txgp1l, gp3, gp34 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Lys32-Pro187 |
| Accession | P07141 |
| Calculated Molecular Weight | 19.0 kDa |
| Observed Molecular Weight | 17-25 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <2 ng/mL.The specific activity of recombinant mouse M-CSF is approximately >5 x 105 IU/mg. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Macrophage colony-stimulating factor 1（M-csf）is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. |
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