Recombinant Mouse METAP2 Protein (His Tag) (PKSM040634)
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For research use only.
| Synonyms | 4930584B20Rik, A930035J23Rik, AI047573, AL024412, Amp2, AU014659, Mnpep, p67, p67eIF2 |
| Species | Mouse |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Ala 2-Tyr 478 |
| Accession | O08663 |
| Calculated Molecular Weight | 54.3 kDa |
| Observed Molecular Weight | 60 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE). The specific activity is > 15 pmol/min/¦Ìg |
| Form | Liquid |
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol |
| Background | METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis. |
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