Recombinant NGF/NGFB Monoclonal Antibody (AN300217P)
For research use only.
| Verified Samples |
Verified Samples in WB: U-87 MG in IF: HeLa |
| Dilution | WB: 1:1000；ICC/IF: 1:100-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ICC/IF |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human NGF / NGFB protein |
| Abbre | NGF |
| Synonyms | HSAN, NGF, Beta-NGF, HSAN5, NGFB, Beta-Nerve Growth Factor, NGF-B, Beta nerve growth factor, Beta NGF, MGC161426, MGC161428, Nerve growth factor, Nerve growth factor (beta polypeptide), Nerve growth factor beta, Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NID67 |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
27 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Endosome lumen. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience |
| Clone | B135 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
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