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Recombinant Osteopontin Monoclonal Antibody

Cat:E-AB-81496
Manual MSDS

Price: $ 260

Price: $ 160

Size:
100μL 50μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Rat;Hamster
  • Applications: WB;IHC-P
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hela,CHO-K1,C6,Jurkat
Verified Samples in IHC:Human colon cancer
Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Western Blot Operation Guide
Clonality Rabbit Monoclonal
Immunogen A synthetic peptide of human Osteopontin
Abbre Osteopontin
Synonyms BNSP;BSPI;ETA-1;OPN;Osteopontin;SPP1
Swissprot
Calculated MW 35kDa
Observed MW 35kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted.
Concentration 300 μg/mL
Buffer 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method Affinity Purified
Research Areas Cancer; Cardiovascular; Signal Transduction; Stem Cells
Clone No. R08-1C5
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.