Recombinant PSGL-1/CD162/SELPLG Monoclonal Antibody (AN300442P)
For research use only.
| Verified Samples |
Verified Samples in IHC:paraffin-embedded human tonsil paraffin-embedded human spleen |
| Dilution | WB 1:1000, IHC-P 1:200-1:1000, ICC/IF 1:50, FC 1:200-1:500, IP 1:20-1:50 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | IHC-P, FCM |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human PSGL-1/CD162/SELPLG Protein |
| Abbre | SELPLG |
| Synonyms | P selectin glycoprotein ligand, P-selectin glycoprotein ligand, CD 162, CD162, CD162 antigen, CLA, Cutaneous lymphocyte associated associated antigen, P selectin glycoprotein ligand 1, P selectin glycoprotein ligand 1 precursor, P-selectin glycoprotein ligand 1, PSGL 1, PSGL1, PSGL-1, Selectin P ligand, SELPL, SELPLG, PEN-5 |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW |
250 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed on neutrophils, monocytes and most lymphocytes. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Affinity Purification |
| Research Areas | Immunology, Cardiovascular |
| Clone | B368 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011] |
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