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Recombinant Rat CD73/NT5E Protein (His Tag)

Cat:PKSR030462
Manual MSDS

Price: $ 447

Price: $ 257

Size:
50μg 10μg
Quantity:
  • Expression Host: HEK293 Cells
  • Accession: P21588
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Product Details
Synonyms CD73;E5NT;Nucleotidase;NT5E;ecto-5'-nucleotidase;5'-Nucleotidase
Species Rat
Expression Host HEK293 Cells
Sequence Trp29-Lys549
Accession P21588
Calculated Molecular Weight 59.0 kDa
Observed Molecular Weight 70-90 kDa
Tag C-His
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Reconstitution Not Applicable
Background CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.