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Recombinant Rat CD73/NT5E Protein (His Tag)

  • Cat.No.:PKSR030462

  • Expression host: Human Cells

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Description

Synonyms CD73; E5NT;Nucleotidase; NT5E;ecto-5'-nucleotidase;5'-Nucleotidase
Species Rat
Expression_host Human Cells
Sequence Trp29-Lys549
Accession P21588
Mol_Mass 59 kDa
AP_Mol_Mass 70-90 kDa
Tag C-His

Properties

Purity > 90% as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice.Upon receipt, store it immediately at<-20°C.
Formulation Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Reconstitution Please refer to the printed manual for detailed information.

Background

CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

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